Trusted by 100+ scientists
from Leading Biopharmaceutical Companies and Academic Institutions
At the smallest scale,
for the highest throughput
S.NEST® 2.0 is a culturing system that monitors cell metabolism in real-time and provides effective mixing in both 96 and 24-well plates. By integrating monitoring, mixing, and incubation into a single instrument, S.NEST significantly reduces time and costs associated with cell culture R&D and manufacturing.
“In-Well” Cell
Metabolic Monitoring
Patented “In-Well”
Mixing
Independent
Incubation Chambers
“In-Well” Cell
Metabolic Monitoring
01. “In-Well” Cell Metabolic Monitoring
↘︎ Mitochondrial respiration and glycolysis insights
↘︎ Real-time, non-invasive, sampling-free monitoring
↘︎ Measurements at 10-min intervals
S.NEST monitors real time DO (dissolved oxygen) and pH in all wells using optical sensors in 96/24-well plates . The data is captured during the entire cell culture period. Our software can further calculate OCR (oxygen consumption rate) and ECAR (extracellular acidification rate) from this data, improving sensitivity to detect metabolic changes earlier.
Application and Tech note
- 24-well plate format monitoring data
- 96-well plate format monitoring data
Patented “In-Well”
Mixing
02. Patented “In-Well”
Mixing
✨ Suspension boost:
↘︎ Homogeneous nutrient distribution
↘︎ Higher oxygen transfer rate
↘︎ 3D growing space
↘︎ Increased cell suspension
Application and Tech note
- Suspension boost data
- Oxygen mass transfer coefficient kLa
Independent
Incubation Chambers
03. Independent
Incubation Chambers
Application and Tech note
- Chamber environment SQP
DO/pH And OCR/ECAR For Cell Metabolic Analysis
Comprehensive insights into cellular function, adaptation, and responses to various physiological
and pathological conditions.
pH monitoring
Lactate accumulate & Metabolic
switch
↘︎ Feed and pH control time point
Conditions
- Cell line: mAb-CHO-K1
- Medium: CD04
- Plate format: 24-well
- Culture volume: 1,400 uL/well
- Mixing rate: 10s/cycle
- Duration: 3 days
DO monitoring
Cell growth rate & Cell viability
↘︎ Cell growth profile
↘︎ Harvest time point
Conditions
- Cell line: mAb-CHO-K1
- Medium: CD04
- Plate format: 24-well
- Culture volume: 1,400 uL/well
- Mixing rate: 10s/cycle
- Duration: 3 days
Software interface
Streamlined Interface,
Data at Your Fingertips!
Navigate effortlessly through our user-friendly software interface, allowing for convenient control of the four incubation chambers. Real-time data on mixing status, culture environment, DO, pH, OCR, and ECAR values provides a comprehensive view of your experiment.
Workstation
Applications
Streamlined cell line development
Early-stage cell clone selection
Miniaturize model for process development
High-throughput drug screening
Analysis of cell metabolism
Higher viability for primary cultures
CHO cell
HEK cell
Hybridoma cell
Stem cell
Cancer cell
iPSC
PBMC
T cell
Spheroid
Organoid
E. coli
Pichia pastoris
Automation-friendly
Designed for maximum productivity, S.NEST is automation-friendly, saving you both time and labor. Its seamless integration with your robotic arm, along with SiLA 2.0 compatibility, makes it a versatile solution. Build your automated station for a streamlined workflow.
Learn more about S.NEST® 2.0
Technical
Specifications
General characteristics
| Description | Value | Unit |
| Dimensions | ||
| Width | 785 | mm |
| Depth | 435 | mm |
| Height | 296 | mm |
| Weight | 49 | kg |
| Electrical characteristics | ||
| Input voltage | 100-240 | Vac |
| Input voltage net tolerance | ±10 | % |
| Input voltage frequency | 50/60 | Hz |
| Max. input current | 11.8-5.4 | A |
| Power mains supply voltage fluctuations | ±10 | A |
| Installation category | CAT ll | - |
| Input fuse type | 250VAC, 16A, time-lag | - |
Basic configuration
| Description | Value | Unit |
| Incubation | ||
| Temperature control range | RT+5 - 45±0.2 | °C |
| CO2 level control range | 1-20±0.3 | % |
| Humidity monitoring range | 0-100±5(at 37°C) | % |
| Culture | ||
| Mixing rate (24-well plate) | 10-50s±5 | % |
| Mixing rate (96-well plate) | 10-50s±5 | % |
| Working volume (24-well plate) | 1,000-1,600 | μL |
| Working volume (96-well plate) | 150-200 | μL |
| Working volume (384-well plate) | 25-80 | μL |
| Sensing | ||
| DO measurement range | 0-100±5** | % |
| pH measurement range | 6-8±0.2 | - |
| Sampling rate | ≥10 | min |
| * Each value above is specified with one standard deviation from its mean (M±1SD) | ||
| ** Once the reference DO (defined as 100%) has been set, the DO readout may vary between 100 +/- 10% due to the fluctuation of oxygen levels in ambient air. | ||
